The Experimental Frog is an enemy that appears in Crash Twinsanity. It's seen in Classroom Chaos. It appears in Crash's section of Classroom Chaos in glass cases. It's seen later as an enemy in Cortex's section. They roam around the certain classrooms. Experimental Frogs can be seen in the Green Gem Gallery.

In one of the concept art galleries, it is seen when Cortex, N. Brio and N. Gin escape away from Madame Amberley, Cortex bringing Victor and Moritz and next to N. Brio there is an experimental frog, which it seems that he created it (because they both have bolts in their heads) or just they encountered one, however it will remain as a mystery.